Discovery and Potential of SNP Markers in Characterization of Tunisian Olive Germplasm

Diversity

Imen Rekik Hakim¹  Naziha Grati Kammoun¹  Emna Makhloufi¹ 

[1] Institut de l'Olivier, P.O. Box 1087, 3018 Sfax, Tunisia; E-Mails:

关键词: cultivar genotyping;    genetic diversity;    Olea europaea L.;    SNP markers;   

DOI  :  10.3390/d2010017

来源: mdpi

【 摘 要 】

Single Nucelotide Polymorphisms (SNPs) have become the most widely used markers in many current genetic applications. Here we report the discovery of nine new SNPs in olives by direct partial sequencing of two genes (OEX and OEW) in sixteen Tunisian cultivars. The SNP markers were then used to genotype 24 olive cultivars and assess the level of genetic diversity. Power of discrimination of SNP markers was then compared to that of microsatellites (SSRs). A combination of SSR and SNP markers was finally proposed that can be used for cultivars identification in juvenile step or for oil traceability.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.