Sensors | |
A Disposable Organophosphorus Pesticides Enzyme Biosensor Based on Magnetic Composite Nano-Particles Modified Screen Printed Carbon Electrode | |
Ning Gan1  Xin Yang1  Donghua Xie2  Yuanzhao Wu1  | |
[1] The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering of Ningbo University, Ningbo, 315211, China; E-Mails:;The Technical Center of Ningbo Entry-Exit Inspection and Quarantine Bureau, Ningbo, 315200, China; E-Mail: | |
关键词: Fe3O4/Au composite nanoparticles; carbon nanotubes; nano-ZrO2; acetyl-cholinesterase; organophosphorus pesticides; prussian blue; screen-printed carbon electrodes; enzyme biosensor; | |
DOI : 10.3390/s100100625 | |
来源: mdpi | |
【 摘 要 】
A disposable organophosphorus pesticides (OPs) enzyme biosensor based on magnetic composite nanoparticle-modified screen printed carbon electrodes (SPCE) has been developed. Firstly, an acetylcholinesterase (AChE)-coated Fe3O4/Au (GMP) magnetic nanoparticulate (GMP-AChE) was synthesized. Then, GMP-AChE was absorbed on the surface of a SPCE modified by carbon nanotubes (CNTs)/nano-ZrO2/prussian blue (PB)/Nafion (Nf) composite membrane by an external magnetic field. Thus, the biosensor (SPCE│CNTs/ZrO2/PB/Nf│GMP-AChE) for OPs was fabricated. The surface of the biosensor was characterized by scanning electron micrography (SEM) and X-ray fluorescence spectrometery (XRFS) and its electrochemical properties were studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The degree of inhibition (
【 授权许可】
CC BY
©2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.
【 预 览 】
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RO202003190055228ZK.pdf | 760KB | download |