Correlation between Antioxidant Activities and Phenolic Contents of Radix Angelicae Sinensis (Danggui)

Molecules

Xican Li¹  Xiaoting Wu¹ 

[1]School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine 510405, Guangzhou, China

关键词: Radix Angelicae Sinensis;    antioxidant;    phenolic;    correlation;   

DOI  :  10.3390/molecules14125349

来源: mdpi

【 摘 要 】

Radix Angelicae Sinensisis (RAS) is one of the most popular traditional Chinese herbal medicines. In the present study, six RAS extracts (i.e., phenolic extract PE, petroleum ether extract PEE, ethyl acetate extract EAE, absolute ethanol extract AEE, 95% ethanol extract 95 EE, and water extract WE) were prepared and their antioxidant activities measured by DPPH (1,1-diphenyl-2-picrylhydrazyl radical), ABTS [2,2′-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid diammonium salt)], Reducing power, •O^2– and lipid peroxidation assays. In general, PE, PEE and EAE had relatively high antioxidant activity, followed by AEE with moderate activity, as compared with 95 EE and WE that had low activity. Their phenolic contents (including total phenolic, ferulic acid, caffeic acid, same as below) were then determined by HPLC or spectrophotometry. The sequence of phenolic contents was roughly identical with that of antioxidant activity. When the values of 1/IC₅₀ of various antioxidant assays were used to evaluate the level of antioxidant of the RAS extracts, (plot between 1/IC₅₀ values and phenolic contents), the correlation coefficient (R) ranged from 0.642 to 0.941, with an average value of 0.839. Significant positive correlations demonstrated that the antioxidant effects of RAS might generally be considered a result of the presence of the phenolic compounds, especially ferulic acid and caffeic acid.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.