Overexpression of Periostin and Lumican in Esophageal Squamous Cell Carcinoma

Cancers

Manoj Kumar Kashyap⁴  Arivusudar Marimuthu⁴  Suraj Peri⁶  Ghantasala S. Sameer Kumar⁴  Harrys K.C. Jacob⁴  Thottethodi Subrahmanya Keshava Prasad⁴  Riaz Mahmood²  K. V. Veerendra Kumar³  M. Vijaya Kumar³  Stephen J. Meltzer⁵  Elizabeth A. Montgomery¹  Rekha V. Kumar⁷ 

[1] Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; E-Mail:;Department of Biotechnology, Kuvempu University, Shimoga District, Karnataka 577451, India; E-Mail:;Department of Surgical Oncology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka 560029, India; E-Mails:;Institute of Bioinformatics, International Technology Park, Bangalore 560066, India; E-Mails:;Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; E-Mail:;Biostatistics and Bioinformatics, Fox Chase Cancer Center, Philadelphia, PA 19111-2497, USA; E-Mail:;Department of Pathology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka 560029, India

关键词: stroma;    extracellular matrix;    DNA microarrays;    epithelial-mesenchymal transition;   

DOI  :  10.3390/cancers2010133

来源: mdpi

【 摘 要 】

To identify biomarkers for early detection for esophageal squamous cell carcinoma (ESCC), we previously carried out a genome-wide gene expression profiling study using an oligonucleotide microarray platform. This analysis led to identification of several transcripts that were significantly upregulated in ESCC compared to the adjacent normal epithelium. In the current study, we performed immunohistochemical analyses of protein products for two candidates genes identified from the DNA microarray analysis, periostin (POSTN) and lumican (LUM), using tissue microarrays. Increased expression of both periostin and lumican was observed in 100% of 137 different ESCC samples arrayed on tissue microarrays. Increased expression of periostin and lumican was observed in carcinoma as well as in stromal cell in the large majority of cases. These findings suggest that these candidates can be investigated in the sera of ESCC patients using ELISA or multiple reaction monitoring (MRM) type assays to further explore their utility as biomarkers.

【 授权许可】

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