Tissue-resident macrophages promote extracellular matrix homeostasis in the mammary gland stroma of nulliparous mice

eLife

Andrew C Nelson¹  Kathryn L Schwertfeger²  Patrice M Witschen³  Ayman M Ibrahim⁴  Thomas S Chaffee⁵  Ying Wang⁵  Danielle N Huggins⁵  Heather L Machado⁵  Rebecca S LaRue⁶ 

[1]Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt

[2]Masonic Cancer Center, University of Minnesota, Minneapolis, United States

[3]Comparative and Molecular Biosciences Graduate Program, University of Minnesota, Minneapolis, United States

[4]Department of Biochemistry and Molecular Biology, Tulane Cancer Center, Tulane School of Medicine, New Orleans, United States

[5]Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, United States

[6]University of Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, United States

关键词: mammary gland;    macrophage;    stroma;    extracellular matrix;   

DOI  :  10.7554/eLife.57438

来源: DOAJ

【 摘 要 】

Tissue-resident macrophages in the mammary gland are found in close association with epithelial structures and within the adipose stroma, and are important for mammary gland development and tissue homeostasis. Macrophages have been linked to ductal development in the virgin mammary gland, but less is known regarding the effects of macrophages on the adipose stroma. Using transcriptional profiling and single-cell RNA sequencing approaches, we identify a distinct resident stromal macrophage subpopulation within the mouse nulliparous mammary gland that is characterized by the expression of Lyve-1, a receptor for the extracellular matrix (ECM) component hyaluronan. This subpopulation is enriched in genes associated with ECM remodeling and is specifically associated with hyaluronan-rich regions within the adipose stroma and fibrous capsule of the virgin mammary gland. Furthermore, macrophage depletion leads to enhanced accumulation of hyaluronan-associated ECM in the adipose-associated stroma, indicating that resident macrophages are important for maintaining homeostasis within the nulliparous mammary gland stroma.

【 授权许可】

Unknown