Development of an Electrochemical Immunosensor for Fumonisins Detection in Foods

Toxins

Mohamad Kamal Abdul Kadir¹ 

[1] Cranfield University, Cranfield Health, Cranfield, Bedfordshire, MK43 0AL, UK;

关键词: fumonisins;    mycotoxins;    screen-printed gold electrode;    electrochemical immunosensor;   

DOI  :  10.3390/toxins2040382

来源: mdpi

【 摘 要 】

An electrochemical affinity sensor for the determination of fumonisins mycotoxins (Fms) using monoclonal antibody modified screen-printed gold electrode with carbon counter and silver-silver chloride pseudo-reference electrode is reported in this work. A direct competitive enzyme-linked immunosorbent assay (ELISA) was initially developed, exhibiting a detection limit of 100 µg·L^-1 for fumonisins. This was then transferred to the surface of a bare gold screen-printed electrode (SPGE) and detection was performed by chronoamperometry, monitoring the reaction of 3,3’,5,5’-Tetramethylbenzidine dihydrochloride (TMB) and hydrogen peroxide (H₂O₂) catalysed by HRP at −100 mV potential vs. onboard Ag-AgCl pseudo-reference electrode. The immunosensor exhibited detection limit of 5 µg·L⁻¹ fumonisins with a dynamic range from 1 µg·L⁻¹–1000 µg·L⁻¹. The sensor also performed well in extracted corn samples.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland