Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts

Toxins

Eleonora Fusi³  Raffaella Rebucci³  Chiara Pecorini³  Anna Campagnoli¹  Luciano Pinotti³  Francesca Saccone³  Federica Cheli³  Stig Purup²  Kristen Sejrsen² 

[1] Università Telematica San Raffaele, Roma, Via di Val Cannuta, 247, 00166 Roma, Italy;Department of Animal Health and Bioscience University of Aarhus, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark;Università degli Studi di Milano, Department of Veterinary Science and Technology for Food Safety, Via Trentacoste 2, I-20134 Milan, Italy;

关键词: ochratoxin A;    α-tocopherol;    DNA damage;    fibroblasts;    swine;   

DOI  :  10.3390/toxins2061265

来源: mdpi

【 摘 要 】

The aims of the current study were to determine the half-lethal concentration of ochratoxin A (OTA) as well as the levels of lactate dehydrogenase release and DNA fragmentation induced by OTA in primary porcine fibroblasts, and to examine the role of α-tocopherol in counteracting its toxicity. Cells showed a dose-, time- and origin-dependent (ear vs. embryo) sensitivity to ochratoxin A. Pre-incubation for 3 h with 1 nM α-tocopherol significantly (P < 0.01) reduced OTA cytotoxicity, lactate dehydrogenase release and DNA damage in both fibroblast cultures. These findings indicate that α-tocopherol supplementation may counteract short-term OTA toxicity, supporting its defensive role in the cell membrane.

【 授权许可】

CC BY   
© 2010 by the authors; licensee MDPI, Basel, Switzerland