Enzymatic Determination of Diglyceride Using an Iridium Nano-Particle Based Single Use, Disposable Biosensor

Sensors

Shu-Yi Hsu²  Brandon Bartling¹  Christina Wang³  Fuh-Sheng Shieu² 

[1] Department of Chemical Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA; E-Mail:;Department of Materials Science and Engineering, National Chung Hsing University, Taichung 40227, Taiwan; E-Mails:;Department of Biological Sciences, College of Arts and Sciences, Cornell University, 147 Goldwin Smith Hall, Ithaca, NY 14853-3201, USA; E-Mail:

关键词: diglyceride;    disposable biosensor;    enzyme;   

DOI  :  10.3390/s100605758

来源: mdpi

【 摘 要 】

A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized at an applied potential of +0.5 V versus the Ag/AgCl reference electrode. The oxidation current was then used to quantify the diglyceride concentration. Optimum enzyme concentrations and the surfactant loading used were established for successful sensor response. Good linear performance was observed over a DG concentration range of 0 to 25 μM in phosphate buffer and bovine serum media.

【 授权许可】

CC BY   
© 2010 by the authors; licensee MDPI, Basel, Switzerland.