Genes | |
454-Pyrosequencing: A Molecular Battiscope for Freshwater Viral Ecology | |
David J. Rooks2  Darren L. Smith2  James E. McDonald2  Martin J. Woodward1  Alan J. McCarthy2  | |
[1] Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, UK; E-Mail:;Microbiology Research Group, School of Biological Sciences, Biosciences Building, University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK; E-Mails: | |
关键词: virome production; viral pathogens; pathogen dissemination; metagenomics; viral ecology; | |
DOI : 10.3390/genes1020210 | |
来源: mdpi | |
【 摘 要 】
Viruses, the most abundant biological entities on the planet, are capable of infecting organisms from all three branches of life, although the majority infect bacteria where the greatest degree of cellular diversity lies. However, the characterization and assessment of viral diversity in natural environments is only beginning to become a possibility. Through the development of a novel technique for the harvest of viral DNA and the application of 454 pyrosequencing, a snapshot of the diversity of the DNA viruses harvested from a standing pond on a cattle farm has been obtained. A high abundance of viral genotypes (785) were present within the virome. The absolute numbers of lambdoid and Shiga toxin (Stx) encoding phages detected suggested that the depth of sequencing had enabled recovery of only
【 授权许可】
CC BY
© 2010 by the authors; licensee MDPI, Basel, Switzerland
【 预 览 】
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RO202003190052919ZK.pdf | 1273KB | download |