The Protective Effects of the Active Fraction of Shaofu Zhuyu Decoction on Hydrogen Peroxide-Induced Oxidative Injury in Vascular Smooth Muscle Cells

Molecules

Li Liu¹  Jin Ao Duan¹  Yu Ping Tang¹  Hong Yue Ma¹  Shu Lan Su¹  Jian Ming Guo¹ 

[1] Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China

关键词: blood stasis syndrome;    oxidative damage;    reactive oxygen species;    apoptosis;    vascular smooth muscle cells;   

DOI  :  10.3390/molecules15085066

来源: mdpi

【 摘 要 】

In this paper, the protective effects of the active fraction (SF-7) from Shaofu Zhuyu decoction (SFZYD) were tested on a hydrogen peroxide (H₂O₂)-induced rat vascular smooth muscle cells (VSMCs) oxidative injury model. This active fraction (SF-7) shows potent antioxidant properties. The cell viability and oxidative damage markers of VSMCs were determined after exposure to H₂O₂ for 16 hours. It was observed that SF-7 significantly increased cell survival and reduced apoptosis of H₂O₂-injured VSMCs. Moreover, SF-7 could markedly increase intracellular superoxide dismutase (SOD) activity and decrease the malondialdehyde (MDA) level in H₂O₂-injured VSMCs, and suppress the generation of intracellular reactive oxygen species (ROS), as well as intracellular Ca²⁺ concentration. Thus, SF-7 exhibits protective effects against H₂O₂-injury on VSMCs, which may be associated with its antioxidant properties. It is suggested that SF may be useful in the treatment of blood stasis syndrome in which oxidative injury is mainly implicated.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.