Chemical Constituents with Free-Radical-Scavenging Activities from the Stem of Microcos paniculata

Molecules

Hua Fan¹  Guang-Zhong Yang¹  Tong Zheng¹  Zhi-Nan Mei¹  Xiang-Ming Liu¹  Yu Chen¹ 

[1] ¹College of Pharmacy, South Central University for Nationalities, Wuhan 430074, China ²College of Biomedical Engineering, South Central University for Nationalities, Wuhan 430074, China ³College of Chemistry and Material Sciences, South Central University for Nationalities, Wuhan 430074, China

关键词: Microcos paniculata;    free-radical-scavenging activities;    DPPH;    ABTS;    Co (II) EDTA-induced luminol chemiluminescence by flow injection;   

DOI  :  10.3390/molecules15085547

来源: mdpi

【 摘 要 】

The free-radical-scavenging activities of various solvent extracts of Microcos paniculata were evaluated through in vitro model systems, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) and Co (II) EDTA-induced luminol chemiluminescence by flow injection. In all three of these systems the ethyl acetate (EtOAc) extract showed the highest free-radical-scavenging activity compared with the other three (n-BuOH, water and petroleum ether) extracts. Free-radical-scavenging assay-guided chromatographic separation of the EtOAc extract, using a normal-phase and reverse-phase silica gel column chromatography yielded five compounds: a new triterpene named methyl 3b-O-p-hydroxy-E-cinnamoyloxy-2a,23-dihydroxyolean-12-en-28-oate (1), whose spectral data are presented for the first time, together with four known compounds, epicatechin (2), 3-trans-feruloyl maslinic acid (3), maslinic acid (4) and sucrose (5). All of the compounds were isolated from Microcos paniculata for the first time. The compounds were identified by spectroscopic methods. Among them, compound 2 displayed significant free-radical-scavenging activity which is similar to that of standard antioxidant ascorbic acid (V_C) and therefore may be a promising natural antioxidant.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.