Inhibitory Effect of Indigo Naturalis on Tumor Necrosis Factor-α-Induced Vascular Cell Adhesion Molecule-1 Expression in Human Umbilical Vein Endothelial Cells

Molecules

Hsin-Ning Chang¹  Jong-Hwei Su Pang¹  Sien-Hung Yang¹  Chi-Feng Hung¹  Chi-Hsin Chiang¹  Tung-Yi Lin¹ 

[1] ¹Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, 222 Mai Chin Road, Keelung 204, Taiwan

关键词: psoriasis;    indigo naturalis;    human umbilical vein endothelial cell;    vascular cell adhesion-1;    c-Jun;   

DOI  :  10.3390/molecules15096423

来源: mdpi

【 摘 要 】

The use of indigo naturalis to treat psoriasis has proved effective in our previous clinical studies. The present study was designed to examine the anti-inflammatory effect of indigo naturalis in primary cultured human umbilical vein endothelial cells (HUVECs). Pretreatment of cells with indigo naturalis extract attenuated TNF-α-induced increase in Jurkat T cell adhesion to HUVECs as well as decreased the protein and messenger (m)RNA expression levels of vascular cell adhesion molecule-1 (VCAM-1) on HUVECs. Indigo naturalis extract also inhibited the protein expression of activator protein-1 (AP-1)/c-Jun, a critical transcription factor for the activation of VCAM-1 gene expression. Since the reduction of lymphocyte adhesion to vascular cells by indigo naturalis extract could subsequently reduce the inflammatory reactions caused by lymphocyte infiltration in the epidermal layer and help to improve psoriasis, this study provides a potential mechanism for the anti-inflammatory therapeutic effect of indigo naturalis extract in psoriasis.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.