Sensors | |
Development of an Electrochemical Biosensor for the Detection of Aflatoxin M1 in Milk | |
Nathalie Paniel1  Antonio Radoi2  | |
[1] id="af1-sensors-10-09439">Laboratoire IMAGES EA 4218, Bâtiments S, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, Fran | |
关键词: electrochemical immunosensor; aflatoxin M1; mycotoxin; milk; horseradish peroxidase (HRP); superparamagnetic nanoparticles; | |
DOI : 10.3390/s101009439 | |
来源: mdpi | |
【 摘 要 】
We have developed an electrochemical immunosensor for the detection of ultratrace amounts of aflatoxin M1 (AFM1) in food products. The sensor was based on a competitive immunoassay using horseradish peroxidase (HRP) as a tag. Magnetic nanoparticles coated with antibody (anti-AFM1) were used to separate the bound and unbound fractions. The samples containing AFM1 were incubated with a fixed amount of antibody and tracer [AFM1 linked to HRP (conjugate)] until the system reached equilibrium. Competition occurs between the antigen (AFM1) and the conjugate for the antibody. Then, the mixture was deposited on the surface of screen-printed carbon electrodes, and the mediator [5-methylphenazinium methyl sulphate (MPMS)] was added. The enzymatic response was measured amperometrically. A standard range (0, 0.005, 0.01, 0.025, 0.05, 0.1, 0.25, 0.3, 0.4 and 0.5 ppb) of AFM1-contaminated milk from the ELISA kit was used to obtain a standard curve for AFM1. To test the detection sensitivity of our sensor, samples of commercial milk were supplemented at 0.01, 0.025, 0.05 or 0.1 ppb with AFM1. Our immunosensor has a low detection limit (0.01 ppb), which is under the recommended level of AFM1 [0.05 μg L-1 (ppb)], and has good reproducibility.
【 授权许可】
CC BY
© 2010 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190052228ZK.pdf | 481KB | download |