Evaluation on Anti-hepatitis Viral Activity of Vitis vinifer L

Molecules

Tao Liu¹  Jun Zhao¹  Haibo Li¹ 

[1] ¹College of Public Health, Xinjiang Medical University, Urumqi, 830011, China ²Xinjiang Key Laboratory for Research and Development of Uighur Medicine, Institute of Materia Medica of Xinjiang, Urumqi, 830002, China ³Nantong Center for Disease Control and Prevention, Nantong, 226007, China

关键词: Vitis vinifer L;    anti-hepatitis viral activity;    orthogonal design;   

DOI  :  10.3390/molecules15107415

来源: mdpi

【 摘 要 】

Suosuo grape (Vitis vinifer L) is traditionally used as a therapeutic agent for measles and hepatitis by the ethnic Uighurs. This work aimed to investigate the anti-HBV effect of total triterpene (VTT), total flavonoids (VTF) and total polysaccharides (VTP) from Suosuo grape, and their synergistic effects were also tested. The viral antigens of cellular secretion, HBsAg and HBeAg, were determined by enzyme linked immunosorbent assay (ELISA).The quantity of HBV-DNA released in the supernatant was assayed by real-time PCR. It was found that it effectively suppressed the secretion of HBsAg and HBeAg from HepG2.2.15 cells in a dose-dependent manner, as well as the HBV DNA. The results of orthogonal design experiment showed that the combination of VTT 20 μg/mL, VTF 50 μg/mL and VTP 50 μg/mL had the best optimistic inhibitory effects on HBeAg secretion.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.