Anti-Tumor Activity of a Novel Protein Obtained from Tartary Buckwheat

International Journal of Molecular Sciences

Xiaona Guo¹  Kexue Zhu¹  Hui Zhang¹ 

[1] State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi-214122, Jiangsu, China; E-Mails:

关键词: tartary buckwheat;    protein;    breast cancer cells;    apoptosis;    antitumor;   

DOI  :  10.3390/ijms11125201

来源: mdpi

【 摘 要 】

TBWSP31 is a novel antitumor protein that was isolated from tartary buckwheat water-soluble extracts. The objective of this paper was to investigate the anti-proliferative effects of TBWSP31 on breast cancer Bcap37cells and to explore its possible mechanism. After treatment of Bcap37 cells with TBWSP31, typical apoptotic morphological changes were observed by inverted microscopy and scanning electron microscopy (SEM), such as detachment from the culture plate, change to a round shape, cell shrinkage, the absence of obvious microvilli, plasma membrane blebbing, and formation of apoptotic bodies. Cell-cycle analysis revealed that treatment with TBWSP31 resulted in a G₀/G₁ arrest and prevented the cells from growing from G₀/G₁ phase to S phase, which was most prominent at 48 h. The expression of bcl-2 and Fas were detected quantitatively by FCM, which showed that TBWSP31 induced-apoptosis may be involved with the participation of Fas and bcl-2. These results suggest that TBWSP31 is a potential antitumor compound and that apoptosis induced by TBWSP31 is a key antitumor mechanism.

【 授权许可】

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