The Chitinolytic Activities of Streptomyces sp. TH-11

International Journal of Molecular Sciences

Kim-Chi Hoang⁴  Tzu-Hsuan Lai¹  Chung-Sheng Lin³  Ying-Tsong Chen² 

[1] Departments of Bioengineering, Tatung University, Taipei 104, Taiwan; E-Mail:;Institute of Genomics and Bioinformatics, National Chung Hsin University, TaiChung City 402, Taiwan; E-Mail:;School of Medicine, Chung Shan Medical University and Department of Internal Medicine, Chung Shan Medical University Hospital, TaiChung City 402, Taiwan; E-Mail:;General Education Center, Ta Hwa Institute of Technology, HsinChu 307, Taiwan; E-Mail:

关键词: chitin;    Streptomyces;    chitinase;   

DOI  :  10.3390/ijms12010056

来源: mdpi

【 摘 要 】

Chitin is an abundant biopolymer composed of units of N-acetyl-D-glucosamine linked by β-1,4 glycosidic bonds. Chitin is the main component of the shells of mollusks, the cell wall of fungi and yeast and of the exoskeleton of crustaceans and insects. The degradation of chitin is catalyzed by chitinases that occur in a wide range of organisms. Among them, the chitinases from microorganisms are extremely important for the degradation and recycling of the carbon and nitrogen trapped in the large amount of insoluble chitin in nature. Streptomyces sp. TH-11 was isolated from the sediment of the Tou-Chien River, Taiwan. The chitinolytic enzyme activities were detected using a rapid in-gel detection method from the cell-free preparation of the culture medium of TH-11. The chitinolytic enzyme activity during prolonged liquid culturing was also analyzed by direct measurement of the chitin consumption. Decomposition of the exoskeleton of shrimps was demonstrated using electron microscopy and atomic force microscopy.

【 授权许可】

CC BY   
© 2011 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.