International Journal of Molecular Sciences | |
Effects of the Molecular Weight and the Degree of Deacetylation of Chitosan Oligosaccharides on Antitumor Activity | |
Jae Kweon Park1  Mi Ja Chung1  Ha Na Choi2  | |
[1]Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do 420-743, Korea | |
关键词: chitosan oligosaccharides; antitumor activity; MALDI-TOF MS; molecular weight; degree of deacetylation; | |
DOI : 10.3390/ijms12010266 | |
来源: mdpi | |
【 摘 要 】
Effects of the degree of deacetylation (DDA) and the molecular mass of chitosan oligosaccharides (CTS-OS), obtained from the enzymatic hydrolysis of high molecular weight chitosan (HMWC), on antitumor activity was explored. The DDA and molecular weights of CTS-OS were determined by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-TOF MS) analysis. The CTS-OS were found to be a mixture of mainly dimers (18.8%), trimers (24.8%), tetramers (24.9%), pentamers (17.7%), hexamers (7.1%), heptamers (3.3%), and octamers (3.4%). The CTS-OS were further fractionated by gel-filtration chromatography into two major fractions: (1) COS, consisting of glucosamine (GlcN)n, n = 3–5 with DDA 100%; and (2) HOS, consisting of (GlcN)5 as the minimum residues and varying number of
【 授权许可】
CC BY
© 2011 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190050831ZK.pdf | 472KB | download |