Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering

Materials

Carlos Mota¹  Dario Puppi¹  Dinuccio Dinucci¹  Cesare Errico¹  Paulo Bártolo² 

[1] Laboratory of Bioactive Polymeric Materials for Biomedical and Environmental Applications (BIOlab), Department of Chemistry and Industrial Chemistry, University of Pisa, via Vecchia Livornese 1291, 56010 San Piero a Grado (Pi), Italy; E-Mails:;Centre for Rapid and Sustainable Product Development, Centro Empresarial da Marinha Grande, Rua de Portugal—Zona Industrial, 2430-028 Marinha Grande, Portugal; E-Mail:

关键词: additive manufacturing;    electrospinning;    scaffold;    tissue engineering;   

DOI  :  10.3390/ma4030527

来源: mdpi

【 摘 要 】

This research activity was aimed at the development of dual-scale scaffolds consisting of three-dimensional constructs of aligned poly(ε-caprolactone) (PCL) microfilaments and electrospun poly(lactic-co-glycolic acid) (PLGA) fibers. PCL constructs composed by layers of parallel microsized filaments (0/90° lay-down pattern), with a diameter of around 365 μm and interfilament distance of around 191 μm, were produced using a melt extrusion-based additive manufacturing technique. PLGA electrospun fibers with a diameter of around 1 μm were collected on top of the PCL constructs with different thicknesses, showing a certain degree of alignment. Cell culture experiments employing the MC3T3 murine preosteoblast cell line showed good cell viability and adhesion on the dual-scale scaffolds. In particular, the influence of electrospun fibers on cell morphology and behavior was evident, as well as in creating a structural bridging for cell colonization in the interfilament gap.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.