Transformation of Geniposide into Genipin by Immobilized β-Glucosidase in a Two-Phase Aqueous-Organic System

Molecules

Yi-Shun Yang¹  Tong Zhang¹  Shai-Cheng Yu¹  Yue Ding¹  Li-Ying Zhang¹  Chen Qiu¹ 

[1] ¹Experiment Center of Teaching & Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China ²College of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China

关键词: immobilized β-glucosidase;    geniposide;    genipin;    aqueous-organic two-phase system;   

DOI  :  10.3390/molecules16054295

来源: mdpi

【 摘 要 】

Genipin is the bioactive compound of geniposide and a natural cross-linking agent. In order to improve the preparation process of genipin, the hydrolysis of geniposide to genipin by immobilized β-glucosidase in an aqueous-organic two-phase system was studied. β-Glucosidase was immobilized by the crosslinking-embedding method using sodium alginate as the carrier. The optimum reaction temperature, pH value and time were 55 °C, 4.5 and 2.5 h, respectively. To reduce genipin hydrolysis and byproduct production the reaction was carried out in an aqueous-organic two-phase system comprising ethyl acetate and sodium acetate buffer. The product was analyzed by HPLC, UV, IR, and NMR. The yield of genipin was 47.81% and its purity was over 98% (HPLC).

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.