Validation of a high performance liquid chromatography (HPLC) method for the quality control of the Traditional Chinese Medicine (TCM) formulation, Traditional Chinese formula Huanglianjiedutang (HLJDT)

Dynamic Chiropractic

Baolin Bian¹  Agnieszka Lower-Nedza² 

[1] Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences 100700 Beijing, China.;Institute of Pharmaceutical Sciences, Pharmacognosy, Karl-Franzens University Graz 8010 Graz, Austria.

关键词: High performance liquid chromatography (HPLC);    quantification;    validation;    huanglianjiedutang (HLJDT);    geniposide;    berberine;    crocin;    baicalin.;   

DOI  :  10.5897/JMPR11.086

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The aim of this study was to optimize ahigh performance liquid chromatography (HPLC) method for the simultaneous quantification of the four main compoundsgeniposide, berberine, baicalin and crocinpresent in theTraditional Chinese formula Huanglianjiedutang (HLJDT). This formula contains Coptidis Rhizoma, Radix Scutellariae, Cortex Phellodendri and Fructus Gardeniaein a ratio of 1.5: 1: 1: 1.5.The decoction of the formula was prepared in the traditional Chinese way. The most suitable boiling time for the decoction turned out to be 60 min. Validation of the method was performed to demonstrate its reproducibility, precision, stability, repeatability, and recovery rate.Excellent behaviours over the investigated concentrations were observed with the values of RSD ≤ 5%. For the estimation of the recovery rate, half of the herbs were boiled together with the pure compounds. As crocin turned out to be an unstable compound its recovery rate could not be determined.The optimized HPLC method provides an accurate and reproducible analysis for the simultaneous determination of geniposide, berberine, baicalin, and crocin in the formulation HLJDT which is a very important tool for the quality control of this Traditional Chinese Medicine (TCM) formula.

【 授权许可】

Unknown