Interaction of Human Serum Album and C60 Aggregates in Solution

International Journal of Molecular Sciences

Maoyong Song¹  Shufang Liu¹  Junfa Yin¹ 

[1]State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 10085, China

关键词: nC₆₀;    HSA;    interaction;    protein;    fluorescence;   

DOI  :  10.3390/ijms12084964

来源: mdpi

【 摘 要 】

An important property of C₆₀ in aquatic ecotoxicology is that it can form stable aggregates with nanoscale dimensions, namely nC₆₀. Aggregation allows fullerenes to remain suspended for a long time, and the reactivity of individual C₆₀ is substantially altered in this aggregate form. Herein, we investigated the interaction of nC₆₀ and human serum album (HSA) using the methods of fluorescence, fluorescence dynamics, circular dichroism (CD), and site marker competitive experiments. We proposed a binding model consistent with the available experimental results for the interactions of nC₆₀ with HSA. During the interaction process, the structure and conformation of HSA were affected, leading to functional changes of drug binding sites of HSA.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.