In Vivo and in Vitro Anti-Inflammatory Activity of Neorogioltriol, a New Diterpene Extracted from the Red Algae Laurencia glandulifera

Marine Drugs

Rim Chatter¹  Rym Ben Othman³  Sameh Rabhi³  Maria Kladi²  Safa Tarhouni¹  Constantinos Vagias²  Vassilios Roussis²  Lamia Guizani-Tabbane³ 

[1] Unit of Biotoxines, Pasteur Institut of Tunis, 13, Place Pasteur, B.P. 74. 1002 Tunis-Belvedere, Tunisia; E-Mails:;Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece; E-Mails:;Laboratory of Immunopathology, Vaccinology and Molecular Genetics (LIVGM), WHO Collaborating Center for Research and Training in Leishmaniasis and International Associated Laboratory (LIA-CNRS), Pasteur Institut of Tunis, 13, Place Pasteur, B.P. 74. 1002 Tunis-Belvedere, Tunisia; E-Mails:

关键词: anti-inflammatory;    neorogioltriol;    Laurencia;    TNFα;    NF-κB;    NO;    COX-2;    carrageenan;   

DOI  :  10.3390/md9071293

来源: mdpi

【 摘 要 】

Neorogioltriol is a tricyclic brominated diterpenoid isolated from the organic extract of the red algae Laurencia glandulifera. In the present study, the anti-inflammatory effects of neorogioltriol were evaluated both in vivo using carrageenan-induced paw edema and in vitro on lipopolysaccharide (LPS)-treated Raw264.7 macrophages. The in vivo study demonstrated that the administration of 1 mg/kg of neorogioltriol resulted in the significant reduction of carregeenan-induced rat edema. In vitro, our results show that neorogioltriol treatment decreased the luciferase activity in LPS-stimulated Raw264.7 cells, stably transfected with the NF-κB-dependent luciferase reporter. This effect on NF-κB activation is not mediated through MAPK pathways. The inhibition of NF-κB activity correlates with decreased levels of LPS-induced tumor necrosis factor-alpha (TNFα) present in neorogioltriol treated supernatant cell culture. Further analyses indicated that this product also significantly inhibited the release of nitric oxide and the expression of cyclooxygenase-2 (COX-2) in LPS-stimulated Raw264.7 cells. These latter effects could only be observed for neorogioltriol concentrations below 62.5 μM. To our knowledge, this is the first report describing a molecule derived from Laurencia glandulifera with anti-inflammatory activity both in vivo and in vitro. The effect demonstrated in vitro may be explained by the inhibition of the LPS-induced NF-κB activation and TNFα production. NO release and COX-2 expression may reinforce this effect.

【 授权许可】

CC BY   
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