Influence of Porcine Intervertebral Disc Matrix on Stem Cell Differentiation

Journal of Functional Biomaterials

Denise Salzig²  Alexandra Schmiermund²  Elke Gebauer¹  Hans-Lothar Fuchsbauer¹ 

[1] Department of Chemical Engineering and Biotechnology, University of Applied Sciences, 64297 Darmstadt, Germany; E-Mails:;Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstraße 14, 35390 Giessen, Germany; E-Mails:

关键词: nucleus pulposus extract;    gelatin;    mesenchymal stem cells;    transglutaminase;   

DOI  :  10.3390/jfb2030155

来源: mdpi

【 摘 要 】

For back disorders, cell therapy is one approach for a real regeneration of a degenerated nucleus pulposus. Human mesenchymal stem cells (hMSC) could be differentiated into nucleus pulposus (NP)-like cells and used for cell therapy. Therefore it is necessary to find a suitable biocompatible matrix, which supports differentiation. It could be shown that a differentiation of hMSC in a microbial transglutaminase cross-linked gelatin matrix is possible, but resulted in a more chondrocyte-like cell type. The addition of porcine NP extract to the gelatin matrix caused a differentiation closer to the desired NP cell phenotype. This concludes that a hydrogel containing NP extract without any other supplements could be suitable for differentiation of hMSCs into NP cells. The NP extract itself can be cross-linked by transglutaminase to build a hydrogel free of NP atypical substrates. As shown by side-specific biotinylation, the NP extract contains molecules with free glutamine and lysine residues available for the transglutaminase.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.