In vitro and in vivo Biocompatibility of Alginate Dialdehyde/Gelatin Hydrogels with and without Nanoscaled Bioactive Glass for Bone Tissue Engineering Applications

Materials

Ulrike Rottensteiner¹  Bapi Sarker²  Dominik Heusinger²  Diana Dafinova¹  Subha N. Rath¹  Justus P. Beier¹  Ulrich Kneser¹  Raymund E. Horch¹  Rainer Detsch²  Aldo R. Boccaccini² 

[1] Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich Alexander University Erlangen-Nuremberg, 91054 Erlangen, Germany; E-Mails:;Institute of Biomaterials, Department of Materials Science and Engineering, Friedrich Alexander University Erlangen-Nuremberg, 91058 Erlangen,Germany; E-Mails:

关键词: alginate dialdehyde;    gelatin;    nano-Bioglass^®;    tissue engineering;    biocompatibility;    mesenchymal stem cells;   

DOI  :  10.3390/ma7031957

来源: mdpi

【 摘 要 】

In addition to good mechanical properties needed for three-dimensional tissue engineering, the combination of alginate dialdehyde, gelatin and nano-scaled bioactive glass (45S5) is supposed to combine excellent cellular adhesion, proliferation and differentiation properties, good biocompatibility and predictable degradation rates. The goal of this study was to evaluate thein vitro and in vivo biocompatibility as a first step on the way to its use as a scaffold in bone tissue engineering. In vitro evaluation showed good cell adherence and proliferation of bone marrow derived mesenchymal stem cells seeded on covalently crosslinked alginate dialdehyde-gelatin (ADA-GEL) hydrogel films with and without 0.1% nano-Bioglass^®(nBG). Lactate dehydrogenase (LDH)- and mitochondrial activity significantly increased in both ADA-GEL and ADA-GEL-nBG groups compared to alginate. However, addition of 0.1% nBG seemed to have slight cytotoxic effect compared to ADA-GEL. In vivo implantation did not produce a significant inflammatory reaction, and ongoing degradation could be seen after four weeks. Ongoing vascularization was detected after four weeks. The good biocompatibility encourages future studies using ADA-GEL and nBG for bone tissue engineering application.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland