Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats

Sensors

Kim E. Sapsford¹  Samantha Spindel¹  Travis Jennings²  Guoliang Tao²  Robert C. Triulzi²  W. Russ Algar³ 

[1] Division of Biology, Office of Science and Engineering Laboratories, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993, USA; E-Mail:;eBioscience, Inc., 10255 Science Center Drive, San Diego, CA 92121, USA; E-Mails:;Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory, 4555 Overlook Avenue, S.W., Washington, DC 20375, USA; E-Mail:

关键词: quantum dot (QD);    nanocrystal (NC);    semiconductor;    bioconjugation;    sensor;    multiplex;    immunoassay;    sulfhydryl chemistry;   

DOI  :  10.3390/s110807879

来源: mdpi

【 摘 要 】

The simultaneous detection of two analytes, chicken IgY (IgG) and Staphylococcal enterotoxin B (SEB), in the single well of a 96-well plate is demonstrated using luminescent semiconductor quantum dot nanocrystal (NC) tracers. The NC-labeled antibodies were prepared via sulfhydryl-reactive chemistry using a facile protocol that took <3 h. Dose response curves for each target were evaluated in a single immunoassay format and compared to Cy5, a fluorophore commonly used in fluorescent immunoassays, and found to be equivalent. Immunoassays were then performed in a duplex format, demonstrating multiplex detection in a single well with limits of detection equivalent to the single assay format: 9.8 ng/mL chicken IgG and 7.8 ng/mL SEB.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.