Flow Injection Spectrophotometric Determination of N-Acetyl-L-cysteine as a Complex with Palladium(II)

Molecules

Josipa Giljanović¹  Mia Brkljača¹ 

[1] ¹Department of Analytical Chemistry, Faculty of Chemistry and Technology, Teslina 10/V, 21000 Split, Croatia ²Department of Mediterranean Agriculture and Aquaculture, University of Zadar, Mihovila Pavlinovića bb, 23000 Zadar, Croatia

关键词: flow-injection;    spectrophotometry;    N-Acetyl-L-cysteine;    palladium;    complexometry;   

DOI  :  10.3390/molecules16097224

来源: mdpi

【 摘 要 】

We describe a new method using flow-injection analysis with spectro-photometric detection, suitable for the determination of N-acetyl-L-cysteine (NAC). The proposed method is appropriate for the determination of NAC in reaction with Pd²⁺ ions in the concentration range from 1.0 × 10⁻⁵ mol L⁻¹ to 6.0 × 10⁻⁵ mol L⁻¹. The detection limit NAC was 5.84 × 10⁻⁶ mol L⁻¹ and the recorded relative standard deviation of the method is in the range from 1.67 to 4.11%. NAC and Pd²⁺ form complexes of Pd²⁺:NAC molar ratios of 1:1 and 1:2, depending on the ratio of their analytical concentrations. The cumulative conditional stability constant for the Pd(NAC)₂²⁺ complex is β₁₂' = 2.69 × 10⁹ L² mol⁻². The proposed method was compared with the classic spectrophotometric determination of NAC, using the same reagent, PdCl₂, and had shown certain advantages: a) shorter analysis time; b) the use of smaller volumes of sample and reagents, which make the proposed method cheaper and faster for NAC determination in real samples without sample pretreatment.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.