A Conductometric Indium Oxide Semiconducting Nanoparticle Enzymatic Biosensor Array

Sensors

Dongjin Lee²  Janet Ondrake¹ 

[1] Department of Mechanical Engineering, Ohio Northern University, 525 South Main St., Ada, OH 45810, USA; E-Mail:;Department of Mechanical Engineering, University of Minnesota, 111 Church St. S.E., Minneapolis, MN 55455, USA; E-Mail:

关键词: biosensor array;    nanoparticle;    conductometric sensor;    microsensor array;    glucose sensor;   

DOI  :  10.3390/s111009300

来源: mdpi

【 摘 要 】

We report a conductometric nanoparticle biosensor array to address the significant variation of electrical property in nanomaterial biosensors due to the random network nature of nanoparticle thin-film. Indium oxide and silica nanoparticles (SNP) are assembled selectively on the multi-site channel area of the resistors using layer-by-layer self-assembly. To demonstrate enzymatic biosensing capability, glucose oxidase is immobilized on the SNP layer for glucose detection. The packaged sensor chip onto a ceramic pin grid array is tested using syringe pump driven feed and multi-channel I–V measurement system. It is successfully demonstrated that glucose is detected in many different sensing sites within a chip, leading to concentration dependent currents. The sensitivity has been found to be dependent on the channel length of the resistor, 4–12 nA/mM for channel lengths of 5–20 μm, while the apparent Michaelis-Menten constant is 20 mM. By using sensor array, analytical data could be obtained with a single step of sample solution feeding. This work sheds light on the applicability of the developed nanoparticle microsensor array to multi-analyte sensors, novel bioassay platforms, and sensing components in a lab-on-a-chip.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.