Mechanistic Studies of the Anti-Ulcerogenic Activity and Acute Toxicity Evaluation of Dichlorido-Copper(II)-4-(2-5-Bromo-benzylideneamino)ethyl) Piperazin-1-ium Phenolate Complex against Ethanol-Induced Gastric Injury in Rats

Molecules

Muhammad Saleh Salga¹  Hapipah Mohd Ali¹  Mahmood Ameen Abdullah¹  Siddig Ibrahim Abdelwahab¹  Pouya Davish Hussain¹ 

[1]¹Department of Chemistry, University of Malaya, 50603 Kuala Lumpur, Malaysia[2] height:6px

[3]"> ²Department of Molecular Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia[4] height:6px

[5]"> ³Department of Pharmacy, University of Malaya, 50603 Kuala Lumpur, Malaysia

关键词: Schiff bases;    acute toxicity;    anti-ulcer activity;    mechanism;    cytokines;   

DOI  :  10.3390/molecules16108654

来源: mdpi

【 摘 要 】

The compound dichlorido-copper(II)-4-(2-5-bromobenzylideneamino)ethyl) piperazin-1-ium phenolate (CuLBS) was synthesized, characterized and screened for acute toxicity and protective activity against ethanol-induced gastric mucosal injury in rats. Gross microscopic lesions, biochemical and immunological parameters and histochemcial staining of glycogen storage were taken into consideration. Oral administration of CuLBS (30 and 60 mg/Kg) for two weeks dose-dependently flattened gastric mucosa, significantly increased gastric mucus and total acidity, compared with control group (P < 0.01). Serum levels of liver enzymes aspartate (AST) and alanine transaminases (ALT), pro-inflammatory (IL-6 and TNF-α) and anti-inflammatory (IL-10) cytokines in the rats exposed to ethanol induced ulceration have been altered. Administration of CuLBS showed considerable (P < 0.05) protection against ulceration by modulating the acute alterations of cytokines AST, ALT and stomach glycogen. Interestingly, CuLBS did not interfere with the natural release of nitric oxide. CuLBS alone (60 mg/Kg) did not exhibit any ulcerogenic effect as assessed using Adami’s scoring scale. An acute toxicity study showed that rats treated with CuLBS (1,000 and 2,000 mg/Kg) manifested no abnormal signs. These findings therefore, suggested that the gastroprotective activity of CuLBS might contribute in modulating the inflammatory cytokine-mediated oxidative damage to gastric mucosa.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.