Molecular Systematics of Polygonum minus Huds. Based on ITS Sequences

International Journal of Molecular Sciences

Hamidun Bunawan¹  Chee Yen Choong²  Badrul Munir Md-Zain²  Syarul Nataqain Baharum¹ 

[1] Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia; E-Mails:;School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia; E-Mails:

关键词: ITS;    phylogenetics;    plastid DNA;    Polygonum minus;    trnL-trnF;   

DOI  :  10.3390/ijms12117626

来源: mdpi

【 摘 要 】

Plastid trnL-trnF and nuclear ribosomal ITS sequences were obtained from selected wild-type individuals of Polygonum minus Huds. in Peninsular Malaysia. The 380 bp trnL-trnF sequences of the Polygonum minus accessions were identical. Therefore, the trnL-trnF failed to distinguish between the Polygonum minus accessions. However, the divergence of ITS sequences (650 bp) among the Polygonum minus accessions was 1%, indicating that these accessions could be distinguished by the ITS sequences. A phylogenetic relationship based on the ITS sequences was inferred using neighbor-joining, maximum parsimony and Bayesian inference. All of the tree topologies indicated that Polygonum minus from Peninsular Malaysia is unique and different from the synonymous Persicaria minor (Huds.) Opiz and Polygonum kawagoeanum Makino.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.