Hepatoprotective Activity of Cichorium endivia L. Extract and Its Chemical Constituents

Molecules

Chao-Jie Chen¹  An-Jun Deng¹  Chang Liu¹  Rui Shi¹  Hai-Lin Qin¹ 

[1] ¹New Drug Safety Evaluation Center, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, No. 1 Xiannongtan Street, Beijing 100050, China ²Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, No. 1 Xiannongtan Street, Beijing 100050, China

关键词: Cichorium endivia L.;    hepatoprotective activity;    oxidaitive damage;    2-furanmethanol-(5'→11)-1;    3-cyclopentadiene-[5;    4-c]-1H-cinnoline;    kaempferol;   

DOI  :  10.3390/molecules16119049

来源: mdpi

【 摘 要 】

The objective of the present study was to investigate the in vitro and in vivo hepatoprotective properties of Cichorium endivia L. extract (CEE), and to identify its chemical constituents. CEE significantly blocked the oxidative stress and cytotoxicity induced by tert-butyl hydroperoxide (t-BHP) in HepG2 cells. Meanwhile, oral administration of CEE to mice before the treatment of t-BHP exhibited a markedly protective effect by lowering serum levels of ALT and AST, inhibiting the changes in liver biochemistry including MDA, SOD, GSH and GST, as well as ameliorating the liver injuries according to the histopathological observations. According to the acute oral toxicity test, the LD₅₀ of CEE was greater than 5,000 mg/kg, which demonstrates that the CEE can be considered practically non-toxic. Phytochemical analysis of CEE showed the presence of five compounds identified as 2-furanmethanol-(5'→11)-1,3-cyclopentadiene-[5,4-c]-1H-cinnoline, which is a new cinnoline derivative derived from a natural source but not synthesis, 2-phenylethyl-β-D-glucopyranoside, kaempferol-3-O-β-D-glucoside, kaempferol, and adenosine. In the ORAC assay, CEE and its constituents kaempferol and kaempferol-3-O-β-D-glucoside had considerable antioxidant potency. Taken together, CEE protects hepatic tissue from oxidative damage in vitro and in vivo, potentially due to its phenolic substances, and does not cause acute oral toxicity, which suggests that CEE may be a valid and safe remedy to cure liver disease.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.