ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin

Biosensors

Zafar Hussain Ibupoto¹  Syed Muhammad Usman Ali²  Kimleang Khun²  Chan Oeurn Chey²  Omer Nur² 

[1] Department of Science and Technology, Campus Norrköping, Linköping University, Norrköping SE-60174, Sweden;

关键词: zinc oxide nanorods;    enzyme penicillinase;    potentiometric nanosensors;    N-5-azido-2-nitrobenzoyloxysuccinimide;    nanodevices;   

DOI  :  10.3390/bios1040153

来源: mdpi

【 摘 要 】

In this study, we have successfully demonstrated the fabrication of a biosensor based on well aligned single-crystal zinc oxide (ZnO) nanorods which were grown on gold coated glass substrate using a low temperature aqueous chemical growth (ACG) method. The ZnO nanorods were immobilized with penicillinase enzyme using the physical adsorption approach in combination with N-5-azido-2-nitrobenzoyloxysuccinimide (ANB-NOS) as cross linking molecules. The potentiometric response of the sensor configuration revealed good linearity over a large logarithmic concentration range from 100 µM to 100 mM. During the investigations, the proposed sensor showed a good stability with high sensitivity of ~121 mV/decade for sensing of penicillin. A quick electrochemical response of less than 5 s with a good selectivity, repeatability, reproducibility and a negligible response to common interferents such as Na¹⁺, K¹⁺, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins, was observed.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.