Sulfated-Polysaccharide Fraction from Red Algae Gracilaria caudata Protects Mice Gut Against Ethanol-Induced Damage

Marine Drugs

Renan Oliveira Silva³  Geice Maria Pereira dos Santos³  Lucas Antonio Duarte Nicolau³  Larisse Tavares Lucetti¹  Ana Paula Macedo Santana¹  Luciano de Souza Chaves²  Francisco Clark Nogueira Barros²  Ana Lྫྷia Ponte Freitas²  Marcellus Henrique Loiola Ponte Souza¹ 

[1] LAFICA—Laboratory of Pharmacology of Inflammation and Cancer, Department of Physiology and Pharmacology, Federal University of Ceará, 60430-270, Fortaleza, CE, Brazil; E-Mails:;Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60455-760, Fortaleza, CE, Brazil; E-Mails:;LAFFEX—Laboratory of Experimental Physiopharmacology, Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí-CMRV, 64202-020, Parnaíba, PI, Brazil; E-Mails:

关键词: polysaccharide;    gastric damage;    ethanol;    nitric oxide;    hydrogen sulfide;   

DOI  :  10.3390/md9112188

来源: mdpi

【 摘 要 】

The aim of the present study was to investigate the gastroprotective activity of a sulfated-polysaccharide (PLS) fraction extracted from the marine red algae Gracilaria caudata and the mechanism underlying the gastroprotective activity. Male Swiss mice were treated with PLS (3, 10, 30 and 90 mg·kg⁻¹, p.o.), and after 30 min, they were administered 50% ethanol (0.5 mL/25 g⁻¹, p.o.). One hour later, gastric damage was measured using a planimeter. Samples of the stomach tissue were also obtained for histopathological assessment and for assays of glutathione (GSH) and malondialdehyde (MDA). Other groups were pretreated with l-NAME (10 mg·kg⁻¹, i.p.), dl-propargylglycine (PAG, 50 mg·kg⁻¹, p.o.) or glibenclamide (5 mg·kg⁻¹, i.p.). After 1 h, PLS (30 mg·kg⁻¹, p.o.) was administered. After 30 min, ethanol 50% was administered (0.5 mL/25g⁻¹, p.o.), followed by sacrifice after 60 min. PLS prevented-ethanol-induced macroscopic and microscopic gastric injury in a dose-dependent manner. However, treatment with l-NAME or glibenclamide reversed this gastroprotective effect. Administration of propargylglycine did not influence the effect of PLS. Our results suggest that PLS has a protective effect against ethanol-induced gastric damage in mice via activation of the NO/K_ATP pathway.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland