Lycium barbarum Polysaccharides Attenuate Cisplatin-Induced Hair Cell Loss in Rat Cochlear Organotypic Cultures

International Journal of Molecular Sciences

Quan Liu¹  Yanqing Li¹  Li Hu² 

[1] Department of Otolaryngology, Eye, Ear, Nose and Throat hospital, Shanghai Medical College of Fudan University, Shanghai 200031, China; E-Mails:;Central Laboratory, Eye, Ear, Nose and Throat hospital, Shanghai Medical College of Fudan University, Shanghai 200031, China; E-Mail:

关键词: Lycium barbarum polysaccharides;    cisplatin;    hair cells;    reactive oxygen species;    mitochondria;   

DOI  :  10.3390/ijms12128982

来源: mdpi

【 摘 要 】

The aim of the present study was to investigate the effects of Lycium barbarum polysaccharides (LBP) on cisplatin-induced hair cell damage in the organ of Corti explant. The neonatal (P2–3) rat organ of Corti explant was exposed to cisplatin (20 μM; 48 h) with or without LBP pretreatment (150 and 600 μg/mL; 24 h). Hair cell loss was indicated by FITC-labeled phalloidin staining. The level of reactive oxygen species (ROS) and alteration of mitochondrial membrane potential (ΔΨ_m) in hair cells were analyzed using fluorescent probes 2′,7′-dichlorofluorescein diacetate and JC-1, respectively. The results showed that LBP significantly attenuated hair cell loss (p < 0.01). Hair cells pretreated with LBP showed significant reduction in ROS production and the decline of ΔΨ_m compared with cisplatin alone group (p < 0.01), indicating the protective effect of LBP on cisplatin-induced hair cell loss. Taken together, these results indicate that LBP was effective in attenuating cisplatin-induced hair cell loss by reducing the production of ROS and maintaining mitochondrial ΔΨ_m.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.