Cleavage of Oligonucleotides Containing a P3’→N5’ Phosphoramidate Linkage Mediated by Single-Stranded Oligonucleotide Templates

Molecules

Kosuke Ramon Ito¹  Tetsuya Kodama¹  Futaba Makimura¹  Noritsugu Hosoki¹  Tomohisa Osaki¹  Ayako Orita¹  Takeshi Imanishi¹ 

[1] ¹Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan ²BNA Inc., 7-7-20 Saito-asagi, Ibaraki, Osaka 567-0085, Japan

关键词: bridged nucleic acids;    DNA sensing;    P3’→N5’ phosphoramidate;   

DOI  :  10.3390/molecules161210695

来源: mdpi

【 摘 要 】

Double-stranded DNA (dsDNA) templates can hybridize to and accelerate cleavage of oligonucleotides containing a P3’→N5’ phosphoramidate (P-N) linkage. This dsDNA-templated cleavage of P-N linkages could be due to conformational strain placed on the linkage upon triplex formation. To determine whether duplex formation also induced conformational strain, we examined the reactivity of the oligonucleotides with a P-N linkage in the presence of single-stranded templates, and compared these reactions to those with dsDNA templates. P-N oligonucleotides that are cleaved upon duplex formation could be used as probes to detect single-stranded nucleic acids.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.