Evaluation of a Centrifuged Double Y-Shape Microfluidic Platform for Simple Continuous Cell Environment Exchange

International Journal of Molecular Sciences

Akihiro Hattori¹ 

[1] Kanagawa Academy of Science and Technology, KSP East 310, 3-2-1 Sakado, Kawasaki 213-0012, Japan; E-Mail:

关键词: microfluidic chip;    Y-shape platform;    medium exchange;    environmental control;    single cell;    centrifugal force;    on-chip cellomics;   

DOI  :  10.3390/ijms13010819

来源: mdpi

【 摘 要 】

We have demonstrated the efficacy of a microfluidic medium exchange method for single cells using passive centrifugal force of a rotating microfluidic-chip based platform. At the boundary of two laminar flows at the gathering area of two microfluidic pathways in a Y-shape, the cells were successfully transported from one laminar flow to the other, without mixing the two microfluidic mediums of the two laminar flows during cell transportation, within 5 s with 1 g (150 rpm) to 36.3 g (900 rpm) acceleration, with 93.5% efficiency. The results indicate that this is one of the most simple and precise tools for exchanging medium in the shortest amount of time.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.