Back to BAC: The Use of Infectious Clone Technologies for Viral Mutagenesis

Viruses

Robyn N. Hall¹  Joanne Meers¹  Elizabeth Fowler² 

[1] School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, Australia;Queensland Agricultural Biotechnology Facility, Ritchie Laboratories, St Lucia, QLD 4067, Australia;

关键词: chromosomes;    artificial;    bacterial;    recombination;    genetic;    mutagenesis;    cloning;    molecular methods;    transposition;    DNA viruses;    infectious clone;   

DOI  :  10.3390/v4020211

来源: mdpi

【 摘 要 】

Bacterial artificial chromosome (BAC) vectors were first developed to facilitate the propagation and manipulation of large DNA fragments in molecular biology studies for uses such as genome sequencing projects and genetic disease models. To facilitate these studies, methodologies have been developed to introduce specific mutations that can be directly applied to the mutagenesis of infectious clones (icBAC) using BAC technologies. This has resulted in rapid identification of gene function and expression at unprecedented rates. Here we review the major developments in BAC mutagenesis in vitro. This review summarises the technologies used to construct and introduce mutations into herpesvirus icBAC. It also explores developing technologies likely to provide the next leap in understanding these important viruses.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.