Chromosome-Specific DNA Repeats: Rapid Identification in Silico and Validation Using Fluorescence in Situ Hybridization

International Journal of Molecular Sciences

Joanne H. Hsu²  Hui Zeng³  Kalistyn H. Lemke³  Aris A. Polyzos³  Jingly F. Weier³  Mei Wang⁴  Anna R. Lawin-O𠆛rien¹  Heinz-Ulrich G. Weier³ 

[1] Department of Fetal Medicine, Whipps Cross University Hospital, Barts Health NHS Trust, London E11 1NR, UK; E-Mail:;Department of Molecular and Cell Biology, University of California, Berkeley (UCB), Berkeley, CA 94720, USA;Life Sciences Division, E.O. Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA; E-Mails:;Department of Diabetes, City of Hope, 1500 Duarte Road, Duarte, CA 91010-3012, USA; E-Mail:

关键词: molecular cytogenetics;    chromosomes;    heterochromatin;    DNA repeats;    data mining;    fluorescence in situ hybridization;    bacterial artificial chromosomes;    DNA probes;   

DOI  :  10.3390/ijms14010057

来源: mdpi

【 摘 要 】

Chromosome enumeration in interphase and metaphase cells using fluorescence in situ hybridization (FISH) is an established procedure for the rapid and accurate cytogenetic analysis of cell nuclei and polar bodies, the unambiguous gender determination, as well as the definition of tumor-specific signatures. Present bottlenecks in the procedure are a limited number of commercial, non-isotopically labeled probes that can be combined in multiplex FISH assays and the relatively high price and effort to develop additional probes. We describe a streamlined approach for rapid probe definition, synthesis and validation, which is based on the analysis of publicly available DNA sequence information, also known as “database mining”. Examples of probe preparation for the human gonosomes and chromosome 16 as a selected autosome outline the probe selection strategy, define a timeline for expedited probe production and compare this novel selection strategy to more conventional probe cloning protocols.

【 授权许可】

CC BY   
© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.