Hypomethylation of cytosine residues in cold-sensitive regions of Cestrum strigilatum (Solanaceae)

Genetics and Molecular Biology

Paula Carolina Paes Guarido²  Adriano Alves De Paula²  Carlos Roberto Maximiano Da Silva²  Carmen Rodriguez¹  André Luís Laforga Vanzela² 

[1] ,Universidade Estadual de Londrina Centro de Ciências Biológicas Departamento de Biologia GeralLondrina PR ,Brazil

关键词: anti-5-methylcytosine;    Cestrum;    chromosomes;    CMA;    heterochromatin;    immunolabeling;   

DOI  :  10.1590/S1415-47572012005000026

来源: SciELO

【 摘 要 】

Heterochromatin comprises a fraction of the genome usually with highly repeated DNA sequences and lacks of functional genes. This region can be revealed by using Giemsa C-banding, fluorochrome staining and cytomolecular tools. Some plant species are of particular interest through having a special type of heterochromatin denominated the cold-sensitive region (CSR). Independent of other chromosomal regions, when biological materials are subjected to low temperatures (about 0 °C), CSRs appear slightly stained and decondensed. In this study, we used Cestrum strigilatum (Solanaceae) to understand some aspects of CSR condensation associated with cytosine methylation levels, and to compare the behavior of different heterochromatin types of this species, when subjected to low temperatures.

【 授权许可】

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