Early Exercise Affects Mitochondrial Transcription Factors Expression after Cerebral Ischemia in Rats

International Journal of Molecular Sciences

Qi Zhang²  Yi Wu²  Hongying Sha¹  Pengyue Zhang²  Jie Jia²  Yongshan Hu² 

[1] State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China; E-Mails:;Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China; E-Mails:

关键词: exercise;    ischemia;    PGC-1;    NRF-1;    COX IV;    neuroprotection;   

DOI  :  10.3390/ijms13021670

来源: mdpi

【 摘 要 】

Increasing evidence shows that exercise training is neuroprotective after stroke, but the underlying mechanisms are unknown. To clarify this critical issue, the current study investigated the effects of early treadmill exercise on the expression of mitochondrial biogenesis factors. Adult rats were subjected to ischemia induced by middle cerebral artery occlusion followed by reperfusion. Expression of two genes critical for transcriptional regulation of mitochondrial biogenesis, peroxisome proliferator-activated receptor coactivator-1 (PGC-1) and nuclear respiratory factor-1 (NRF-1), were examined by RT-PCR after five days of exercise starting at 24 h after ischemia. Mitochondrial protein cytochrome C oxidase subunit IV (COX IV) was detected by Western blot. Neurological status and cerebral infarct volume were evaluated as indices of brain damage. Treadmill training increased levels of PGC-1 and NRF-1 mRNA, indicating that exercise promotes rehabilitation after ischemia via regulation of mitochondrial biogenesis.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.