Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads

Molecules

Bong-Hyun Jun¹  Homan Kang¹  Yoon-Sik Lee¹ 

[1] ¹School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Korea ²Nano Systems Institute and Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-747, Korea ³Department of Chemistry Education, Seoul National University, Seoul 151-747, Korea

关键词: optically-encoded bead;    fluorescence;    quantum dots;    surface-enhanced Raman scattering (SERS);    bead-based assay;    label-free detection;    high-throughput screening;   

DOI  :  10.3390/molecules17032474

来源: mdpi

【 摘 要 】

Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.