An Electrochemical Method to Detect Gamma Glutamyl Transpeptidase

International Journal of Molecular Sciences

Guifang Chen¹  Shengfa Ni²  Sha Zhu²  Jinghua Yang³ 

[1] Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China; E-Mail:;Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China; E-Mails:;Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China; E-Mail:

关键词: gamma glutamyl transpeptidase;    glutathione;    copper ions;    electrochemical;   

DOI  :  10.3390/ijms13032801

来源: mdpi

【 摘 要 】

Gamma glutamyl transpeptidase (GGT) is a transferase, which is of great importance in sustaining intracellular cysteine and glutathione levels. The abnormal expression of GGT is significantly associated with features of many metabolic syndromes (e.g., hepatocellular carcinoma). Therefore, it is essential to develop methods to detect GGT so as to monitor the physiological or pathological phenomena related to this species. In this work, by making use of a complex formed by Cu²⁺ and glutathione, which may exhibit excellent voltammetric response, we have proposed a novel potential electrochemical method for the detection of the enzyme. Results show that in the presence of GGT, the formation of Cu²⁺-glutathione complex on a working electrode will be disrupted, resulting in greatly depressed electrochemical signals. The primary method exhibits some advantages, such as it being fast, cost-efficient, and conveniently operated. It also has the potential to be further developed as an effective method in the quantitative detection of GGT in real samples.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.