Effects of Different Extraction Methods and Conditions on the Phenolic Composition of Mate Tea Extracts

Molecules

Nevena Grujic¹  Zika Lepojevic¹  Branislava Srdjenovic¹  Jelena Vladic¹ 

[1] ¹Department of Pharmacy, Medical Faculty, University of Novi Sad, Serbia ²Department of Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Serbia ³The Institute for the Health Protection of Workers Novi Sad, Serbia

关键词: mate;    chlorogenic acid;    HPLC analysis;    DPPH;    total phenolic content;    total flavonoid content;   

DOI  :  10.3390/molecules17032518

来源: mdpi

【 摘 要 】

A simple and rapid HPLC method for determination of chlorogenic acid (5-O-caffeoylquinic acid) in mate tea extracts was developed and validated. The chromatography used isocratic elution with a mobile phase of aqueous 1.5% acetic acid-methanol (85:15, v/v). The flow rate was 0.8 mL/min and detection by UV at 325 nm. The method showed good selectivity, accuracy, repeatability and robustness, with detection limit of 0.26 mg/L and recovery of 97.76%. The developed method was applied for the determination of chlorogenic acid in mate tea extracts obtained by ethanol extraction and liquid carbon dioxide extraction with ethanol as co-solvent. Different ethanol concentrations were used (40, 50 and 60%, v/v) and liquid CO₂ extraction was performed at different pressures (50 and 100 bar) and constant temperature (27 ± 1 °C). Significant influence of extraction methods, conditions and solvent polarity on chlorogenic acid content, antioxidant activity and total phenolic and flavonoid content of mate tea extracts was established. The most efficient extraction solvent was liquid CO₂ with aqueous ethanol (40%) as co-solvent using an extraction pressure of 100 bar.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.