An Organic/Inorganic Hybrid Membrane as a Solid “Turn-On” Fluorescent Chemosensor for Coenzyme A (CoA), Cysteine (Cys), and Glutathione (GSH) in Aqueous Media

Sensors

Saúl Vallejos¹  Pedro Estévez¹  Saturnino Ibeas¹  Félix C. Garc໚¹  Felipe Serna¹ 

[1] Departamento de Química, Facultad de Ciencias, Universidad de Burgos, Plaza de Misael Bañuelos s/n, E-09001 Burgos, Spain;

关键词: sensory materials;    chemosensor;    fluorogenic sensor;    biomolecules;    sensing biomolecules;   

DOI  :  10.3390/s120302969

来源: mdpi

【 摘 要 】

The preparation of a fluorogenic sensory material for the detection of biomolecules is described. Strategic functionalisation and copolymerisation of a water insoluble organic sensory molecule with hydrophilic comonomers yielded a crosslinked, water-swellable, easy-to-manipulate solid system for water “dip-in” fluorogenic coenzyme A, cysteine, and glutathione detection by means of host-guest interactions. The sensory material was a membrane with gel-like behaviour, which exhibits a change in fluorescence behaviour upon swelling with a water solution of the target molecules. The membrane follows a “turn-on” pattern, which permits the titration of the abovementioned biomolecules. In this way, the water insoluble sensing motif can be exploited in aqueous media. The sensory motif within the membrane is a chemically anchored piperazinedione-derivative with a weakly bound Hg(II). The response is caused by the displacement of the cation from the membrane due to a stronger complexation with the biomolecules, thus releasing the fluorescent sensory moieties within the membrane.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland