Nutrients | |
Current Challenges in Detecting Food Allergens by Shotgun and Targeted Proteomic Approaches: A Case Study on Traces of Peanut Allergens in Baked Cookies | |
Romina Pedreschi1  Jørgen Nørgaard1  | |
[1] European Commission (EC), DG Joint Research Centre (JRC), Institute for Reference Materials and Measurements (IRMM), Geel 2440, Belgium; | |
关键词: data dependent acquisition; peanut; food allergens; proteomics; shotgun; selective reaction monitoring; targeted; | |
DOI : 10.3390/nu4020132 | |
来源: mdpi | |
【 摘 要 】
There is a need for selective and sensitive methods to detect the presence of food allergens at trace levels in highly processed food products. In this work, a combination of non-targeted and targeted proteomics approaches are used to illustrate the difficulties encountered in the detection of the major peanut allergens Ara h 1, Ara h 2 and Ara h 3 from a representative processed food matrix. Shotgun proteomics was employed for selection of the proteotypic peptides for targeted approaches via selective reaction monitoring. Peanut presence through detection of the proteotypic Ara h 3/4 peptides AHVQVVDSNGNR (
【 授权许可】
CC BY
© 2012 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190045550ZK.pdf | 704KB | download |