Electrochemical l-Lactic Acid Sensor Based on Immobilized ZnO Nanorods with Lactate Oxidase

Sensors

Zafar Hussain Ibupoto¹  Syed Muhammad Usman Ali Shah²  Kimleang Khun² 

[1] Department of Science and Technology, Campus Norrköping, Linköping University, SE-60174, Norrköping, Sweden;

关键词: zinc oxide nanorods;    lactate oxidase enzyme;    glutaraldehyde;    potentiometric nanostructured biosensor;    nanodevices;   

DOI  :  10.3390/s120302456

来源: mdpi

【 摘 要 】

In this work, fabrication of gold coated glass substrate, growth of ZnO nanorods and potentiometric response of lactic acid are explained. The biosensor was developed by immobilizing the lactate oxidase on the ZnO nanorods in combination with glutaraldehyde as a cross linker for lactate oxidase enzyme. The potentiometric technique was applied for the measuring the output (EMF) response of l-lactic acid biosensor. We noticed that the present biosensor has wide linear detection range of concentration from 1 × 10⁻⁴–1 × 10⁰ mM with acceptable sensitivity about 41.33 ± 1.58 mV/decade. In addition, the proposed biosensor showed fast response time less than 10 s, a good selectivity towards l-lactic acid in presence of common interfering substances such as ascorbic acid, urea, glucose, galactose, magnesium ions and calcium ions. The present biosensor based on immobilized ZnO nanorods with lactate oxidase sustained its stability for more than three weeks.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland