Antinociceptive and Anti-inflammatory Effects of a Lectin-Like Substance from Clitoria fairchildiana R. Howard Seeds

Molecules

Joana Filomena Magalhães Leite¹  Ana Maria Sampaio Assreuy¹  Mário Rogério Lima Mota¹  Pedro Henrique de Souza Ferreira Bringel¹  Rodrigo Rodrigues e Lacerda¹  Vinícius de Morais Gomes¹  João Batista Cajazeiras¹  Kyria Santiago do Nascimento¹  Hilzeth de Luna Freire Pessôa¹  Carlos Alberto de Almeida Gadelha¹  Plinio Delatorre¹  Benildo Sousa Cavada¹ 

[1] ¹Laboratory of Biologically Actives Molecules, Department of Biochemistry and Molecular Biology, Federal University of Ceará, P.O. Box 6043, CEP 60455-970 Fortaleza, Ceará, Brazil

关键词: Clitoria;    purification;    glycoprotein;    toxicity;    biological activity;   

DOI  :  10.3390/molecules17033277

来源: mdpi

【 摘 要 】

Lectins are proteins that have the ability to bind specifically and reversibly to carbohydrates and glycoconjugates, without altering the structure of the glycosyl ligand. They are found in organisms such as viruses, plants and humans, and they have been shown to possess important biological activities. The objective of this study was to purify and characterize lectins in the seeds of Clitoria fairchildiana, as well as to verify their biological activities. The results indicated the presence of a lectin (CFAL) in the glutelin acid protein fraction, which agglutinated native rabbit erythrocytes. CFAL was purified by column chromatography ion-exchange, DEAE-Sephacel, which was obtained from a peak of protein retained in the matrix by applying 0.5 M NaCl using the step-wise method. Electrophoretic analysis of this lectin in SDS-PAGE indicated a two band pattern protein molecular mass of approximately 100 and 116 kDa. CFAL proved to be unspecific to all carbohydrates/glycoconjugates in common use for the sugar inhibition test. This lectin showed no significant cytotoxicity to human red blood cells. It was observed that CFAL has anti-inflammatory activity in the paw edema induced by carrageenan model, in which a 64% diminution in edema was observed. Antinociceptive effects were observed for CFAL in the abdominal writhing test (induced by acetic acid), in which increasing doses of the lectin caused reduction in the number of contortions by up to 72%. It was concluded that the purified and characterized lectin from the seeds of Clitoria fairchildiana has anti-inflammatory and antinociceptive activity, and is not cytotoxic to human erythrocytes.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.