Differential binding of human blood group Sd(a+) and Sd(a−) Tamm‐Horsfall glycoproteins with Dolichos biflorus and Vicia villosa‐B4 agglutinins

FEBS Letters

Wu, June H.¹  Chen, Chie-Pein²  Watkins, Winifred M.³  Wu, Albert M.²  Chen, Yuen-Yuen²  Song, Shuh-Chyung² 

[1] Department of Microbiology and Immunology, Chang-Gung Medical College, Kwei-san, Tao-yuan, Taiwan;Glycoimmunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang-Gung Medical College, Kwei-san, Tao-yuan 333, Taiwan;Department of Haematology, Royal Postgraduate Medical School, Imperial College of Science, Technology, and Medicine, Du Cane Road, London W12 ONN, UK

关键词: Binding properties of GalNAc specific lectins;    Native and asialo-Tamm-Horsfall glycoproteins;    Sugar inhibitors: Gal;    d-galactopyranose;    Glc;    d-glucopyranose;    GalNAc;    2-acetamido-2-deoxy-d-galactopyranose;    GlcNAc;    2-acetamido-2-deoxy-d-glucopyranose;    Man;    d-mannopyranose;    NeuAc;    sialic acid;    Glycoproteins: gp;    glycoprotein;    THGP;    Tamm-Horsfall glycoprotein;    and asialo THGP;    Asialo OSM;    asialo ovine submandibular gp;    Lectins: VVA−B4;    Vicia villosa-B4 agglutinin;    DBA;    Dolichos biflorus agglutinin;    Jacalin;    Artocarpus integrifolia;    CFT;    Codium fragile subspecies tomentosoides;    QPA;    quantitative precipitin assay;    ELLSA;    enzyme linked lectinosorbent assay;   

DOI  :  10.1016/S0014-5793(98)00617-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding patterns of human blood group Sd(a+) and Sd(a−) Tamm-Horsfall glycoproteins (THGPs) with respect to four GalNAc specific agglutinins were studied by quantitative precipitin assay (QPA) and enzyme linked lectinosorbent assay (ELLSA). Of the native and asialo Sd(a+) and Sd(a−) THGP tested by QPA and ELLSA, only native and asialo Sd(a+) bound well with Dolichos biflorus (DBA) and Vicia villosa-B₄ (VVA-B₄), while Sd(a−) THGP reacted poorly with these two lectins. Neither Sd(a+) nor Sd(a−) THGPs reacted with two other GalNAc α-anomer specific lectins: Codium fragile subspecies tomentosoides and Artocarpus integrifolia. Furthermore, the binding of asialo Sd(a+)THGP-VVA-B₄ and native Sd(a+)THGP-DBA through GalNAcβ→ was confirmed by inhibition assay. These results demonstrate that DBA and VVA-B₄ are useful reagents to differentiate between Sd(a+) and Sd(a−) THGP.

【 授权许可】

Unknown