Development of Eighteen Microsatellite Markers in Anemone amurensis (Ranunculaceae) and Cross-Amplification in Congeneric Species

International Journal of Molecular Sciences

Mingzhou Sun¹  Xiao Yin¹  Fengxue Shi¹  Lin Li¹  Mingrui Li¹  Linfeng Li¹ 

[1] Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun 130024, China;

关键词: Anemone;    A. amurensis;    microsatellite;    polyploidy;    polymorphism information content;   

DOI  :  10.3390/ijms13044889

来源: mdpi

【 摘 要 】

Polyploidy plays an important role in the evolution of plant genomes. To enable the investigation of the polyploidy events within the genus Anemone, we developed eighteen microsatellite markers from the hexaploid species A. amurensis (Ranunculaceae), and tested their transferability in five closely related species. The number of total alleles (N_A) for each resulting locus varied from one to eight. The polymorphism information content (PIC) and Nei’s genetic diversity (N_GD) for these microsatellites ranged from 0.00 to 0.71 and 0.00 to 0.91, respectively. For each population, the N_A was one to seven, and the values of PIC and N_GD varied from 0.00 to 0.84 and 0.00 to 0.95, respectively. In addition, most of these microsatellites can be amplified successfully in the congeneric species. These microsatellite primers provide us an opportunity to study the polyploid evolution in the genus Anemone.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.