Labeling of Anti-MUC-1 Binding Single Chain Fv Fragments to Surface Modified Upconversion Nanoparticles for an Initial in Vivo Molecular Imaging Proof of Principle Approach

International Journal of Molecular Sciences

Anja Hischemöller¹  Claudia Walter¹  Volker Weiler²  Helga Hummel²  Theo Thepen⁴  Michael Huhn⁴  Stephan Barth⁴  Werner Hoheisel⁵  Karen Köhler⁵  Diana Dimova-Landen⁵  Christoph Bremer³  Markus Haase¹ 

[1] Department of Chemistry, University of Osnabrück, Barbarastr. 7, 46069 Osnabrück, Germany; E-Mails:;Philips Research Laboratories Aachen, Weisshausstr. 2, 52066 Aachen, Germany; E-Mails:;Molecular Imaging Group, Department of Clinical Radiology, University Hospital Münster, Waldeyerstr. 1, 48149 Münster, Germany; E-Mail:;Frauenhofer IME, Fockenbeckstr. 6, 52074 Aachen, Germany; E-Mails:;Bayer Technology Services GmbH, Building E41, 51368 Leverkusen, Germany; E-Mails:

关键词: anti-MUC-1 single-chain antibody fragment;    upconversion nanoparticles;    labeling;    in vivo optical molecular imaging;   

DOI  :  10.3390/ijms13044153

来源: mdpi

【 摘 要 】

In vivo optical Imaging is an inexpensive and highly sensitive modality to investigate and follow up diseases like breast cancer. However, fluorescence labels and specific tracers are still works in progress to bring this promising modality into the clinical day-to-day use. In this study an anti-MUC-1 binding single-chain antibody fragment was screened, produced and afterwards labeled with newly designed and surface modified NaYF₄:Yb,Er upconversion nanoparticles as fluorescence reporter constructs. The MUC-1 binding of the conjugate was examined in vitro and in vivo using modified state-of-the-art small animal Imaging equipment. Binding of the newly generated upconversion nanoparticle based probe to MUC-1 positive cells was clearly shown via laser scanning microscopy and in an initial proof of principal small animal optical imaging approach.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.