Cytotoxicity and Glycan-Binding Properties of an 18 kDa Lectin Isolated from the Marine Sponge Halichondria okadai

Toxins

Ryo Matsumoto¹  Yuki Fujii¹  Sarkar M. A. Kawsar¹  Robert A. Kanaly¹  Hidetaro Yasumitsu¹  Yasuhiro Koide¹  Imtiaj Hasan¹  Chihiro Iwahara¹  Yukiko Ogawa³  Chang Hun Im⁴  Shigeki Sugawara⁴  Masahiro Hosono⁴  Kazuo Nitta⁴  Jiharu Hamako²  Taei Matsui² 

[1] Laboratory of Glycobiology and Marine Biochemistry, Department of Genome System Science, Graduate School of NanoBio Sciences, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan;Department of Biology, Fujita Health University, Toyoake, Aichi 470-1192, Japan;Divisions of Functional Morphology and Microbiology, Department of Pharmacy, Faculty of Pharmaceutical Science, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan;Division of Cell Recognition Study, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, 4-4-1 Komatsusima, Aoba-ku, Sendai 981-8558, Japan;

关键词: cytotoxicity;    frontal affinity chromatography technology;    glycoprotein;    Japanese black sponge (Halichondria okadai);    lectin;   

DOI  :  10.3390/toxins4050323

来源: mdpi

【 摘 要 】

A divalent cation-independent lectin—HOL-18, with cytotoxic activity against leukemia cells, was purified from a demosponge, Halichondria okadai. HOL-18 is a 72 kDa tetrameric lectin that consists of four non-covalently bonded 18 kDa subunits. Hemagglutination activity of the lectin was strongly inhibited by chitotriose (GlcNAcβ1-4GlcNAcβ1-4GlcNAc), fetuin and mucins from porcine stomach and bovine submaxillary gland. Lectin activity was stable at pH 4–12 and temperatures lower than 60 °C. Frontal affinity chromatography with 16 types of pyridylaminated oligosaccharides indicated that the lectin had an affinity for N-linked complex-type and sphingolipid-type oligosaccharides with N-acetylated hexosamines and neuramic acid at the non-reducing termini. The lectin killed Jurkat leukemia T cells and K562 erythroleukemia cells in a dose- and carbohydrate-dependent manner.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.